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v2Aa Sequences

Create back-translated DNA sequences from AA sequences.

Create back-translated DNA sequences from AA sequences.

Authentication

AuthorizationBearer

Bearer authentication of the form Bearer <token>, where token is your auth token.

Request

This endpoint expects an object.
aaSequenceIdslist of stringsRequired

IDs of AA sequences to back-translate.

folderIdstringRequired

ID of the folder in which the back-translated sequences will be saved.

avoidedCutsiteEnzymeIdslist of stringsOptional

List of enzyme IDs whose recognition sites will be avoided when creating the back-translated sequence.

codonUsageTableIdstringOptional
ID of the codon usage table representing the target organism.
gcContentenumOptionalDefaults to ANY

The amount of GC content in the optimized sequence. LOW is defined as below 0.33, MEDIUM as 0.33-0.66, and HIGH as above 0.66. If neither gcContent nor gcContentRange is specified, the optimization will default to ANY (0-1). Cannot be specified together with gcContentRange.

Allowed values:
gcContentRangeobjectOptional
Custom GC content range for the optimized sequence, specified as decimal values between 0 and 1. The maximum must be greater than the minimum. Cannot be specified together with gcContent.
hairpinParametersobjectOptional
These parameters are applied in the AvoidHairpins specification in DNAChisel. If hairpinParameters is not specified, hairpins will not be avoided.
methodenumOptionalDefaults to MATCH_CODON_USAGE

The codon optimization algorithm to use. Requires codonUsageTableId to be specified. MATCH_CODON_USAGE selects codons probabilistically based on the organism’s codon usage frequencies. USE_BEST_CODON always selects the most frequently used codon for each amino acid.

Allowed values:
reducedPatternslist of objectsOptional

List of patterns to avoid when creating the back-translated sequence, on the coding strand only.

schemaIdstringOptional
ID of the resulting DNA sequences' schemas
shouldDepleteUridinebooleanOptionalDefaults to false

If not specified, the back-translation will default to false, and mRNA uridine depletion will not be performed.

Response

This endpoint launches a long-running task and returns the Task ID of the launched task. When successful, the task returns the resources of the DNA Sequence entities that were back-translated.

taskIdstringOptional

Errors

400
Bad Request Error